Biological effects of a semiconductor diode laser on human periodontal ligament fibroblasts
최은정, 구기태, 설양조, 이용무, 구영, 류인철, 정종평, 김태일,
소속 상세정보
최은정 ( Choi Eun-Jeong ) - Seoul National University College of Medicine Department of Periodontology
구기태 ( Koo Ki-Tae ) - Seoul National University College of Medicine Department of Periodontology
설양조 ( Seol Yang-Jo ) - Seoul National University College of Medicine Department of Periodontology
이용무 ( Lee Yong-Moo ) - Seoul National University College of Medicine Department of Periodontology
구영 ( Ku Young ) - Seoul National University College of Medicine Department of Periodontology
류인철 ( Rhyu In-Chul ) - Seoul National University College of Medicine Department of Periodontology
정종평 ( Chung Chong-Pyoung ) - Seoul National University College of Medicine Department of Periodontology
김태일 ( Kim Tae-Il ) - Seoul National University College of Medicine Department of Periodontology
KMID : 0363020100400030105
Abstract
Purpose It has been reported that low-level semiconductor diode lasers could enhance the wound healing process. The periodontal ligament is crucial for maintaining the tooth and surrounding tissues in periodontal wound healing. While low-level semiconductor diode lasers have been used in low-level laser therapy, there have been few reports on their effects on periodontal ligament fibroblasts (PDLFs). We performed this study to investigate the biological effects of semiconductor diode lasers on human PDLFs.
Methods Human PDLFs were cultured and irradiated with a gallium-aluminum-arsenate (GaAlAs) semiconductor diode laser of which the wavelength was 810 nm. The power output was fixed at 500 mW in the continuous wave mode with various energy fluencies, which were 1.97, 3.94, and 5.91 J/cm2. A culture of PDLFs without laser irradiation was regarded as a control. Then, cells were additionally incubated in 72 hours for MTS assay and an alkaline phosphatase (ALPase) activity test. At 48 hours post-laser irradiation, western blot analysis was performed to determine extracellular signal-regulated kinase (ERK) activity. ANOVA was used to assess the significance level of the differences among groups (P<0.05).
Results At all energy fluencies of laser irradiation, PDLFs proliferation gradually increased for 72 hours without any significant differences compared with the control over the entire period taken together. However, an increment of cell proliferation significantly greater than in the control occurred between 24 and 48 hours at laser irradiation settings of 1.97 and 3.94 J/cm2 (P<0.05). The highest ALPase activity was found at 48 and 72 hours post-laser irradiation with 3.94 J/cm2 energy fluency (P<0.05). The phosphorylated ERK level was more prominent at 3.94 J/cm2 energy fluency than in the control.
Conclusions The present study demonstrated that the GaAlAs semiconductor diode laser promoted proliferation and differentiation of human PDLFs.
키워드
Alkaline phosphatase; Extracellular signal-regulated kinases; Fibroblasts; Periodontal ligament; Semiconductor diode lasers
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